Informatics Publishing Limited

G. Raveendra Babu ¹, J. Srinivasa Rao ², K. Suresh Kumar ², P. Jayachandra Reddy ³

Affiliations
1 Dr.Reddy’s Laboratories Ltd, Bachupally, Hyderabad, Andhra Pradesh, IN
2 United States Pharmacopeia-India Pvt. Ltd, ICICI Knowledge Park, Turkapally, Shameerpet, Hyderabad, IN
3 KrishnaTeja Pharmacy College, Tirupathi, Chittor (Dt), Andhra Pradesh, IN

Abstract

A novel stability indicating liquid chromatographic assay method was developed and validated as per ICH guide lines for the quantitative estimation of Aripiprazole in tablet formulation. An isocratic reverse phase LC-method was developed using Phenomenex Luna C18, 150 x 4.6mm, 5μm column and a mobile phase comprising of Acetonitrile and Phosphate buffer, 0.05M (40:60 v/v). The detector set at 227nm with flow rate of 1.0mL min^-1. The method is Linear in rage of 25μg mL^-1 to 200 μg mL^-1. The Accuracy of the method was found to be in the range of 99.95% to 100.0%. The mean Inter and Intraday assay Relative Standard deviation (%RSD) were less than 1.0%. The Analyte and mobile phase were stable up to 48hours. The Proposed method was found to be Linear, precise and accurate for the quantitative estimation of Aripiprazole in tablet formulations and can be used for commercial purposes.

Keywords

Aripiprazole, Liquid Chromatography, Stress Degradation and Method Validation.

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J. Srinivasa Rao ¹, J. Naga Mallika ¹, M. Sri Madhu Sri ², G. Raveendra Babu ², M. Prasada Rao ², C. Gopinath ¹

Affiliations
1 Hindu College of Pharmacy, Amravati Road, Guntur, Andhrapradesh, IN
2 MAM College of Pharmacy, Narasaraopet, Guntur (Dt), Andhrapradesh, IN

Abstract

The present study has made an attempt to compare % release of three different brands (Lanzol, Lanzap and Lan) of lansoprazole using USP Type II (Paddle) dissolution Apparatus in three Media (phosphate buffer pH 6.0, 6.8 and 7.4). It has been observed from the dissolution profile that more than 90% of Lansoprazole is released from each capsule at 30.0 minutes time point. The F1 and F2 values of Lanzol Vs Lanzap are 7.02% and 59.87%, Lanzol Vs Lan are 1.51% and 81.99% and Lan Vs Lanzop are 0.38% and 59.98% at pH6.0; The F₁ and F₂ values of Lanzol Vs Lanzap are 7.65% and 55.95%, Lanzol Vs Lan are 3.91% and 69.18%, Lan Vs Lanzop are 3.90% and 61.99% at pH 6.8.The F₁ and F₂ values of Lanzol Vs Lanzap are 4.12% and 67.10%, Lanzol Vs Lan 2.46%, 72.60%, Lan Vs Lanzop 1.70% and 84.53% at pH 7.4. In all three Media % Release pattern is similar; However in pH 7.4 media % Release is about 100% at 45minutes.

Keywords

Lansoprazole, Dissolution, UV Method.

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Ravi Kumar vejendla ¹, G. Hemanth Kumar ¹, G. Raveendra Babu ², J. Srinivasarao ³

Affiliations
1 Sri Indu Institute of Pharmacy, Sheriguda (Village), Ibrahimpatan-501 510, RR Dist., Andhra Pradesh, IN
2 Dr.Reddy’s Laboratories Ltd, Bachupally, Hyderabad, Andhra Pradesh, IN
3 United States Pharmacopeia-India Private Limited, ICICI Knowledge Park, Turkapally, Shameerpet, Hyderabad, IN

Abstract

A novel stability indicating liquid chromatographic assay method was developed and validated as per ICH guidelines for the quantitative estimation of Donepezil in tablet formulation. An isocratic reverse phase LC-method was developed using Zorbax SB C18, 150 x 4.6mm, 5μm column and a mobile phase comprising of a mixture of water: acetonitrile (68:32), pH adjusted to 4.5 with trifluoro acetic acid. The detector set at 229nm with flow rate of 1.0mL min^-1. The method is linear between 5 μg mL^-1 to 25 μg mL^-1 and the limit of detection (LOD) is 0.5 μg mL^-1. The Accuracy of the method was found to be in the range of 99.70% to 100.26%. The mean Inter and Intraday assay Relative Standard deviation (%RSD) were less than 0.69%. The Proposed method was found to be Linear, precise and accurate for the quantitative estimation of Donepezil in tablet formulations and can be used for commercial purposes.

Keywords

Donepezil, Liquid Chromatography, Stress Degradation and Method Validation.

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Nalla Priyanka ¹, P. Brahmeswari ², G. Raveendra Babu ³, M. Sowjanya ⁴, M. Ramayyappa ³

Affiliations
1 Department of Chemistry, A.K.R.G. College of Pharmacy, Nallajerla, Andhra Pradesh, IN
2 Dept. of Pharmacology, Brilliant Group of Institutions, Abdullapaur, R.R. Dist., Hyderabad, Telangana, IN
3 Department of Analysis, A.K.R.G. College of Pharmacy, Nallajerla, Andhra Pradesh, IN
4 Vijaya Teja Degree College, Addanki, Andhra Pradesh, IN

Abstract

The current investigation is started to find the antibacterial and anti-inflammatory activities of the sub shrub Hibiscus hirtus L. The leaves of this conventional shrub were found to possess' different activities which incorporate antibacterial and hostile to helminthic effects. Thus we made an endeavor to perform extraction utilizing ethanol as solvent and soxhlation as a strategy for extraction. The leaves were priorly researched for their pharmacognostic, phytochemical and physical parameters. The antibacterial action of the ethanolic leaf extract was conveyed at various concentrations on strains of gram positive and gram-negative bacteria, for example, Staphylococcus aureus and Escherechia coli individually by utilizing cup and plate technique. The outcomes were outfitted as the zone of inbition acquired around every grouping of the concentrate and were compared with those of standard antibiotic utilized in the analysis, amoxycillin. Anti-inflammatory activity of Hibiscus hirtus L was studied and compared with standard drug, ibuprofen. Ethanollic concentrate of leaves (2.5- 15 mg/ml) and ibuprofen (2.5-15 mg/ml) were utilized to measure the paw edema by plethysmometer and compared with control groups. Hibiscus hirtus L showed critical decrease of paw edema in the chosen model of inflammation for example formalin prompted chronic model of inflammation. Ethanollic concentrate of the plant leaves showed anti- microbial and anti-inflammatory impacts.

Keywords

Hibiscus hirtus L, Anti-inflammatory, Antimicrobial activity, Extraction

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Ayanam Vasavi ¹, Miriyala Mrunalini ¹, Ayanam Vasanthi ¹, G. Raveendra Babu ², M. Sowjanya ³

Affiliations
1 Department of Pharmaceutics, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, IN
2 Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, IN
3 Department of Chemistry, Vijaya Teja Degree College, Addanki - 523201, Andhra Pradesh, IN

Abstract

As a novel drug delivery system, microspheres improve the efficacy of a drug, increase the time of action, lower the number of times in which a dosage form needs to be administered, and to increase patient compliance. Oral administration side effects such as gastric irritation are lessened through the use of microspheres. Ionotropic gelation was used to create HPMC K15M, Guar gum, and Carbopol 934 microspheres with different concentrations of Carbopol 934 polymer. Cross-linking was accomplished with the use of calcium chloride. In order to conduct a systematic evaluation of all the preparations, we performed various tests: morphology, FTIR, DSC, entrapment efficiency, size, microsphere size, and in-vitro drug release. The discrete, free-flowing, and spherical particles of prepared Stavudine microspheres were found. In compliance with standards, the mean particle size was in the range of 72.64 to 95.22 percent. In vitro drug release studies were performed in phosphate buffer solution with a pH of 6.8. As the concentration of sodium alginate and calcium chloride increased, the percentage of drug release was reduced. In the case of F9 formulation, which contained Stavudine, the decreased drug release rate was obtained via carbopol 934(1:3), sodium alginate, and calcium chloride. Conclusively, the present study shows that Stavudine microsphere preparation and formulation F9 are successful. Stavudine microspheres must be prepared in order to preserve an effective drug concentration in serum for a long time, while reducing gastrointestinal irritation.

Keywords

Microspheres, Controlled Release, Stavudine, In-vitro Studies

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Ayanam Vasanthi ¹, Ayanam Vasavi ¹, Miriyala Mrunalini ¹, G. Raveendra Babu ², M. Sowjanya ³

Affiliations
1 Department of Pharmaceutics, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, IN
2 Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, IN
3 Department of Chemistry, Vijaya Teja Degree College, Addanki, Andhra Pradesh, IN

Abstract

Currently, this study focuses on developing immediate-release Fexofenadine hydrochloride tablets. The Fexofenadine HCl tablets have super disintegrants that help to accelerate dissolution and bioavailability. Sodium lauryl sulphate, microcrystalline cellulose as filler, crospovidone, and sodium starch glycolate were used to make the tablets using a direct compression method (2-8 percent). Pre and post compressional parameters were used in the preparation of the tablets. The In-vitro disintegration study shows that as concentration of sodium starch glycolate is increased, there is an increase in the amount of time it takes for the solution to disintegrate, but at the same time, there is a decrease in the amount of time it takes for the solution to disintegrate when the crospovidone level is increased. When combined with the crospovidone tablet formulation, the test found that the resulting tablets broke down in approximately 3 to 6 minutes, with enough force to release the fragments but not to harm the friability of the product. It was found that Fexofenadine hydrochloride tablet, a fast-acting form of treatment for allergic rhinitis, could be formulated in an immediate-release form.

Keywords

Allergic Rhinitis, Crospovidone, Croscarmellose Sodium

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M. Ramayyappa ¹, G. Raveendra Babu ¹, H.S.P. Syloosha ¹, V. Alekya Satyasri ¹, G. premi ¹, Ch. Prudhvi ¹

Affiliations
1 Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh, IN

Abstract

Oils and fats in the diet serve three functions in the human body: as an energy source, a structural component, and as important biological regulators. Lipid peroxidation may be accelerated by repeatedly heating soy oil. Unsaturated fatty acids that have been oxidised may play a role in the aetiology of a variety of diseases. The purpose of this study was to see if repeatedly heating edible oil had any negative consequences on illness development. In this investigation, four different edible oils were heated to their regular boiling point and then heated for another 3 hours at a 1hour interval. The heated samples were subjected to qualitative examination. After analysing the results, it was discovered that saponification values declined as continuous heating, i.e. 3 hours, was applied to all different oils, resulting in low saponification values when compared to 2 hours, 1 hour, and normal boiling point. Lower the saponification value, the more free fatty acids and triglycerides are produced, which may contribute to cardio vascular illness. The acid values grew as the heating progressed, according to the findings. A high acid number suggests that there is a lot of acid in the system. The values of iodine fell as the heating progressed, according to the results. The presence of saturated fatty acids is indicated by a low iodine value. Saturated fatty acids were detrimental to one's health. As the heating progressed, the peroxide levels grew. Deterioration is indicated by an increase in peroxide value. When compared to other oils, palm oil showed a high saponification value, acid value, peroxide value, and low iodine value. Total cholesterol, tri-glycerides, and glucose levels were higher in 3 hours heated oils than in 2 hours, 1 hour, and at normal boiling point in biological tests .

Keywords

Edible oil, Heating, Fatty acids, Deterioration, Peroxide values.

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Nalla Priyanka ¹, M. Sowjanya ², Miriyala Mrunalini ³, Ayinam Vasavi ⁴, G. Raveendra Babu ⁵

Affiliations
1 Department of Pharmaceutical Chemistry, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh., IN
2 Department of Chemistry, Vijaya Teja Degree College, Addanki-523201, Andhra Pradesh ., IN
3 Department of Pharmaceutics, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh ., IN
4 Department of Pharmaceutics, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh., IN
5 Department of Pharmaceutical Analysis, A.K.R.G. College of Pharmacy, Nallajerla, W.G. Dist., Andhra Pradesh., IN

Abstract

The study's goal is to synthesis novel benzodiazepine analogues, some of which are chalcones. The elemental analysis, infrared, and 1H nuclear magnetic resonance examinations examined the structures of the newly produced substances. To test for anti-inflammatory and anti-oxidant properties, all substances bearing a descriptor were evaluated. Here, 1,5-(disubstituted phenyl)-2,4-pentadien-1-one derivatives are created from scratch using a new process. By condensing cinnamaldehyde with different aromatic ketones in the presence of 10% NaOH as a base, 1,5-(disubstituted phenyl)-2,4-pentadien-1-one was produced. 2-(substituted phenyl)-3- styryl-2,3-dihydro-1H-benzodiazepine derivatives were synthesised from 1,5-(disubstituted phenyl)-2,4- pentadien-1-one on cyclization with o-phenylene diamine in the presence of NaOH as base. Carrageenan and formalin-induced rat paw edoema technique was used to evaluate the antioxidant and anti-inflammatory activities of the final synthesised benzodiazepine derivatives. Compared to the usual ascorbic acid and diclofenac sodium, the compounds displayed significant anti-inflammatory effect. This means that nitro, chloro, fluoro, and bromo included electron withdrawing groups, which caused an increase in anti-inflammatory action. Most of the produced drugs had strong anti-inflammatory efficacy .

Keywords

Chalcones, 1,5-benzodiazepines, Anti-inflammatory activity, Anti-oxidant activity.

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